Growth hormone releasing hormone (GRH) kit is too good to use - Database & Sql Blog Articles

EL-C1600N100013-B

WIFI test head can be customized for other specifications

Test - lowercase jpg

Kaixin micro test

Growth Hormone Releasing Hormone (GRH) Kit

Introduction

What is the Growth Hormone Releasing Hormone? Clinically, somatostatin is a 14 amino acid polypeptide first isolated from the rat hypothalamus in 1968. It was found to inhibit the release of growth hormone, hence it is known as a growth hormone release inhibitor. Somatostatin is present in D cells of the thalamic pancreatic region and the gastrointestinal tract, where it can broadly suppress the release of various peptides. As a result, this hormone not only inhibits endocrine and exocrine functions but also affects intestinal motility and gallbladder contraction. In 1977, Ganda and Larsson independently described somatostatin tumors in their own reports.

GRH Kit

Experimental Condition Selection

In ELISA, selecting the right experimental conditions is crucial. Here are some key factors to consider:

(1) Solid Phase Support: Common materials include polyvinyl chloride, polystyrene, polyacrylamide, and cellulose. These can come in the form of plates, tubes, or beads. The most commonly used is a 40-well polystyrene plate. Before use, it’s important to screen the carrier by coating it with an equal amount of antigen under the same conditions and checking for uniform color development and good adsorption performance.

(2) Coated Antibody or Antigen: The antibody or antigen should be of high purity, and the pH is usually maintained between 9.0 and 9.6. Adsorption temperature, time, and protein concentration all affect the results. Typically, incubation at 4°C for 18–24 hours is recommended. The optimal protein concentration is determined by titration—coating different concentrations (e.g., 0.1, 1.0, and 10 μg/ml) and measuring the OD value of the positive sample. The best concentration is usually between 1–10 μg/ml.

(3) Working Concentration of Enzyme-Labeled Antibody: Start with a preliminary titer using direct ELISA. Then, fix other conditions or use the “square matrix method” to accurately determine the optimal dilution in the full experimental system.

(4) Enzyme Substrate and Hydrogen Donor: Choose a safe and cost-effective hydrogen donor that produces a clear color reaction. Some options, like OPD, may have carcinogenic risks and should be handled carefully. TMB and ABTS are currently preferred due to their safety and sensitivity. After the substrate has been applied for about 10–30 minutes, add a strong acid or base to stop the reaction. Always prepare the substrate fresh, especially H₂O₂ before use.

Our company's ELISA kits are designed with high sensitivity, strong specificity, and stable performance. When you purchase our ELISA kit, you gain access to professional enzyme-linked immunoassay services, free technical support, and zero-cost shipping. We also offer excellent after-sales service to ensure your satisfaction. Our ELISA kits guarantee quality, and we provide technical guidance before and after the sale. If any quality issues arise, we offer free returns.

Many customers have concerns when comparing different ELISA kits. At XinFan Bio, we are committed to providing reliable quality assurance, so you can buy with confidence and peace of mind.

Purchase our ELISA kit and enjoy free shipping, enhanced after-sales support, and access to expert enzyme-linked immunoassay services. Our skilled technicians and advanced lab equipment ensure accurate and reliable experimental results. All of our ELISA kits are of high quality, and we provide comprehensive technical support throughout the entire process. If you encounter any quality issues, we offer free returns without hesitation.