5-NT ELISA Kit (fish 5 nucleotidease ELISA)

5-XS0B032M200
LM285DR-2-5

Test samples include serum, plasma, cell supernatant, tissue homogenate, and other related biological fluids.

Name 96-well 48-well Remarks
Microporous Enzyme Plate 8 well × 12 8 holes × 12 2-8 °C
Standard 6 6 -20 °C (incomplete)
Sample dilution 6 mL 3 mL Dilute detection antibody according to the instructions
-HRP 10mL 5mL 2-8°C
Substrate A 6mL 3mL 2-8°C
Substrate B 6mL 3mL 2-8°C
Stop solution 6mL 3mL 2-8°C
20× concentrated wash buffer 25ml 15ml No instructions
1 part 1 no self-sealing 1 piece of no-sealing film, 2 sheets

Equipment and reagents required:

  • 450±10nm filter microplate reader (recommended to preheat before use)
  • High-precision pipette and tips: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL
  • Eppendorf pipette
  • Distilled or deionized water
  • Absorbent cotton or paper
  • 37°C incubator
  • 100ml and 1000ml graduated cylinders
  • Prepare several standard dilution tubes

Service Standards:

  • Free replacement for quality issues
  • Comprehensive technical support
  • Free on-site survey service available
  • We will provide solutions within one working day for any customer inquiries
  • Our after-sales and technical teams are dedicated to ensuring customer satisfaction

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