The details determine success or failure, are you clear about the details of the elisa experiment? -Huaqiang Electronic Network

"A journey of a thousand miles begins with a single foot; a mile of a thousand miles, destroyed by an ant hole"; in the experiment, the details are often the key to determining the success of the experiment, the same,

The ELISA experiment has some small details that cannot be ignored while it is strictly required:
1. The speed of sucking liquid with a gun should not be too fast, so as to avoid air bubbles and make the suction amount inaccurate.
2. After all the liquids have been added, the enzyme plate can be gently shaken on the table for 30 seconds to mix the liquid. It is also possible to use the shaking function of the microplate reader.
3. When the washing liquid is not enough, you can prepare PH7.4, 0.02M phosphate buffer solution by using distilled water, and add 0.1% Tween 20 as the washing liquid. It can be stored for a long time after adding 1/1000 sodium azide.
4. When sucking the liquid, it is necessary to remove the gun with a dose and a quantity close to the required amount to reduce the error.
The details determine success or failure, please don't ignore the details in your experiment. If you have any questions, please feel free to contact us.